The TriVersa NanoMate ® ® is the latest in chip-based electrospray ionization technology from Advion. It combines the benefits of liquid chromatography, mass spectrometry, chip-based infusion, fraction collection, and direct surface analysis into one integrated ion source platform. It allows scientists to obtain more information from complex samples than LC/MS alone. For a full inventory of TriVersa NanoMate product notes, application notes, posters, presentations and more, visit our digital resource library.
The TriVersa NanoMate ® is the only automated ion source platform that allows high throughput infusion experiments for small or large analytes in applications ranging from shotgun lipidomics, to non-covalent interaction studies (NCI), to protein characterization. Operating in Mode 1: Infusion, the system utilizes the ® providing long, stable sprays, and eliminating sample-to-sample carryover. Typical Applications.
Lipidomics, Metabolomics. Non-covalent Interaction Studies (NCI). Top-down Protein Identification and Characterization.
QA/QC of Antibodies. Glycan Analysis Benefits. Zero sample-to-sample carryover due to one tip-one sample-one sprayer workflow. Highly efficient, automated nano-electrospray.
Long, stable sprays from µL of sample. High sample throughput from 96, 384 or 1536 well plates.
. Zimmermann, Hartmut F; Rieth, Jochen 2007-03-01 High throughput robotic systems have been used since the 1990s to carry out biochemical assays in microtiter plates. However, before the application of such systems in industrial fermentation process development, some important specific demands should be taken into account. These are sufficient oxygen supply, optimal growth temperature, minimized sample evaporation, avoidance of contaminations, and simple but reliable process monitoring. A fully automated solution where all these aspects have been taken into account is presented. Wang, Wenhui; Liu, Xinyu; Gelinas, Danielle; Ciruna, Brian; Sun, Yu 2007-09-12 As an important embodiment of biomanipulation, injection of foreign materials (e.g., DNA, RNAi, sperm, protein, and drug compounds) into individual cells has significant implications in genetics, transgenics, assisted reproduction, and drug discovery. This paper presents a microrobotic system for fully automated zebrafish embryo injection, which overcomes the problems inherent in manual operation, such as human fatigue and large variations in success rates due to poor reproducibility.
Based on computer vision and motion control, the microrobotic system performs injection at a speed of 15 zebrafish embryos (chorion unremoved) per minute, with a survival rate of 98% (n = 350 embryos), a success rate of 99% (n = 350 embryos), and a phenotypic rate of 98.5% (n = 210 embryos). The sample immobilization technique and microrobotic control method are applicable to other biological injection applications such as the injection of mouse oocytes/embryos and Drosophila embryos to enable high-throughput biological and pharmaceutical research. Muntener, Michael; Patriciu, Alexandru; Petrisor, Doru; Mazilu, Dumitru; Bagga, Herman; Kavoussi, Louis; Cleary, Kevin; Stoianovici, Dan 2011-01-01 Objectives To introduce the development of the first magnetic resonance imaging (MRI)-compatible robotic system capable of automated brachytherapy seed placement. Methods An MRI-compatible robotic system was conceptualized and manufactured.
The entire robot was built of nonmagnetic and dielectric materials. The key technology of the system is a unique pneumatic motor that was specifically developed for this application. Various preclinical experiments were performed to test the robot for precision and imager compatibility.
Results The robot was fully operational within all closed-bore MRI scanners. Compatibility tests in scanners of up to 7 Tesla field intensity showed no interference of the robot with the imager. Precision tests in tissue mockups yielded a mean seed placement error of 0.72 ± 0.36 mm. Conclusions The robotic system is fully MRI compatible. The new technology allows for automated and highly accurate operation within MRI scanners and does not deteriorate the MRI quality.
We believe that this robot may become a useful instrument for image-guided prostate interventions. PMID:17169653. Jones, P; Watson, A; Davies, M; Stubbings, S 1992-01-01 We describe here the integration of image analysis and robotics to produce a fully automated colony picking/plate handling system. Biological tests were performed to verify its performance in terms of sterilisation and accuracy of picking.
The machine was then used by a single operative to pick a 36,000 clone cDNA library in approximately 42 hrs over 5 days. Images PMID:1408762. Purcell, Royal 1991-01-01 Discussion of indexing focuses on the possibilities of fully automated indexing.
Topics discussed include controlled indexing languages such as subject heading lists and thesauri, free indexing languages, natural indexing languages, computer-aided indexing, expert systems, and the need for greater creativity to further advance automated indexing. Kertesz, Vilmos; Van Berkel, Gary J 2010-01-01 A fully automated liquid extraction-based surface sampling device utilizing an Advion NanoMate chip-based infusion nanoelectrospray ionization system is reported. Analyses were enabled for discrete spot sampling by using the Advanced User Interface of the current commercial control software.
This software interface provided the parameter control necessary for the NanoMate robotic pipettor to both form and withdraw a liquid microjunction for sampling from a surface. The system was tested with three types of analytically important sample surface types, viz., spotted sample arrays on a MALDI plate, dried blood spots on paper, and whole-body thin tissue sections from drug dosed mice. The qualitative and quantitative data were consistent with previous studies employing other liquid extraction-based surface sampling techniques. The successful analyses performed here utilized the hardware and software elements already present in the NanoMate system developed to handle and analyze liquid samples. Implementation of an appropriate sample (surface) holder, a solvent reservoir, faster movement of the robotic arm, finer control over solvent flow rate when dispensing and retrieving the solution at the surface, and the ability to select any location on a surface to sample from would improve the analytical performance and utility of the platform. Camper, DeMarco V.; Viola, Ronald E.
2009-01-01 Obtaining highly purified proteins is essential to begin investigating their functional and structural properties. The steps that are typically involved in purifying proteins can include an initial capture, intermediate purification, and a final polishing step. Completing these steps can take several days and require frequent attention to ensure success. Our goal was to design automated protocols that will allow the purification of proteins with minimal operator intervention.
Separate methods have been produced and tested that automate the sample loading, column washing, sample elution and peak collection steps for ion-exchange, metal affinity, hydrophobic interaction and gel filtration chromatography. These individual methods are designed to be coupled and run sequentially in any order to achieve a flexible and fully automated protein purification protocol.
PMID:19595984. Andersen, David; Rasmussen, Brian; Linnet, Kristian 2012-05-01 A fully automated setup was developed for preparing whole blood samples using a Tecan Evo workstation. By integrating several add-ons to the robotic platform, the flexible setup was able to prepare samples from sample tubes to a 96-well sample plate ready for injection on liquid chromatography-mass spectrometry using several preparation techniques, including protein precipitation, solid-phase extraction and centrifugation, without any manual intervention. Pipetting of a known aliquot of whole blood was achieved by integrating a balance and performing gravimetric measurements. The system was able to handle 1,073 of 1,092 (98.3%) samples of whole blood from forensic material, including postmortem samples, without any need for repeating sample preparation. Only three samples required special treatment such as dilution.
The addition of internal and calibration standards were validated by pipetting a solution of Orange G and measuring the weight and absorbance. Internal standard (20 µL) was added in a multi-pipetting sequence with an accuracy of 99.9% and imprecision (coefficient of variation) of 1.6%. Calibration standards were added with high accuracy at volumes as low as 6.00 µL (±0.21 µL). The general setup of the offline sample preparation and key validation parameters of a quantitative analysis of Δ(9)-tetrahydrocannabinol is presented. 1985-04-01 Industrial robots are now proven technology in a variety of applications including welding, materials handling, spray painting, machine loading and assembly.
However, to fully realize the potential of these universal manipulators, 'intelligence' needs to be added to the industrial robot. This involves adding sensory capability and machine intelligence to the controls. The 'intelligence' may be added externally or as integral components of the robot. These new 'intelligent robots' promise to greatly enhance the versatility of the robot for factory applications. The purpose of this paper is to present a brief review of the techniques and applications of intelligent robots for factory automation and to suggest possible designs for the intelligent robot of the future. Jiang, Hao; Ouyang, Zheng; Zeng, Jianing; Yuan, Long; Zheng, Naiyu; Jemal, Mohammed; Arnold, Mark E 2012-06-01 Biological sample dilution is a rate-limiting step in bioanalytical sample preparation when the concentrations of samples are beyond standard curve ranges, especially when multiple dilution factors are needed in an analytical run.
We have developed and validated a Microsoft Excel-based robotic sample preparation program (RSPP) that automatically transforms Watson worklist sample information (identification, sequence and dilution factor) to comma-separated value (CSV) files. The Freedom EVO liquid handler software imports and transforms the CSV files to executable worklists (.gwl files), allowing the robot to perform sample dilutions at variable dilution factors. The dynamic dilution range is 1- to 1000-fold and divided into three dilution steps: 1- to 10-, 11- to 100-, and 101- to 1000-fold. The whole process, including pipetting samples, diluting samples, and adding internal standard(s), is accomplished within 1 h for two racks of samples (96 samples/rack).
This platform also supports online sample extraction (liquid-liquid extraction, solid-phase extraction, protein precipitation, etc.) using 96 multichannel arms. This fully automated and validated sample dilution and preparation process has been applied to several drug development programs.
The results demonstrate that application of the RSPP for fully automated sample processing is efficient and rugged. The RSPP not only saved more than 50% of the time in sample pipetting and dilution but also reduced human errors.
The generated bioanalytical data are accurate and precise; therefore, this application can be used in regulated bioanalysis. Felder, R A 1991-01-01 Robotic technology is going to have a profound impact on the clinical laboratory of the future.
Faced with increased pressure to reduce health care spending yet increase services to patients, many laboratories are looking for alternatives to the inflexible or 'fixed' automation found in many clinical analyzers. Robots are being examined by many clinical pathologists as an attractive technology which can adapt to the constant changes in laboratory testing. Already, laboratory designs are being altered to accommodate robotics and automated specimen processors.
However, the use of robotics and computer intelligence in the clinical laboratory is still in its infancy. Successful examples of robotic automation exist in several laboratories.
Investigators have used robots to automate endocrine testing, high performance liquid chromatography, and specimen transportation. Large commercial laboratories are investigating the use of specimen processors which combine the use of fixed automation and robotics. Robotics have also reduced the exposure of medical technologists to specimens infected with viral pathogens. The successful examples of clinical robotics applications were a result of the cooperation of clinical chemists, engineers, and medical technologists. At the University of Virginia we have designed and implemented a robotic critical care laboratory. Initial clinical experience suggests that robotic performance is reliable, however, staff acceptance and utilization requires continuing education. We are also developing a robotic cyclosporine which promises to greatly reduce the labor costs of this analysis.
The future will bring lab wide automation that will fully integrate computer artificial intelligence and robotics. Specimens will be transported by mobile robots. Specimen processing, aliquotting, and scheduling will be automated.(ABSTRACT TRUNCATED AT 250 WORDS). Dobrotin, B. M.; Hansen, G. Free download indesign portable cs4. C.; Rennels, D.
1977-01-01 The replacement of the driver with an automatic system which could perform the functions of guiding and routing a vehicle with a human's capability of responding to changing traffic demands was discussed. The problem was divided into four technological areas; guidance, routing, computing, and communications. It was determined that the latter three areas being developed independent of any need for fully automated urban traffic. A guidance system that would meet system requirements was not being developed but was technically feasible. Summers, Ronald M. 2016-01-01 OBJECTIVE Automated analysis of abdominal CT has advanced markedly over just the last few years.
Advion Triversa Nanomate
Fully automated assessment of organs, lymph nodes, adipose tissue, muscle, bowel, spine, and tumors are some examples where tremendous progress has been made. Computer-aided detection of lesions has also improved dramatically. CONCLUSION This article reviews the progress and provides insights into what is in store in the near future for automated analysis for abdominal CT, ultimately leading to fully automated interpretation. PMID:27101207. 1985-01-01 The introduction of automation into the laboratory of biology seems to be unavoidable.
But at which cost, if it is necessary to purchase a new machine for every new application? Fortunately the same image processing techniques, belonging to a theoretic framework called Mathematical Morphology, may be used in visual inspection tasks, both in car industry and in the biology lab. Since the market for industrial robotics applications is much higher than the market of biomedical applications, the price of image processing devices drops, and becomes sometimes less than the price of a complete microscope equipment. The power of the image processing methods of Mathematical Morphology will be illustrated by various examples, as automatic silver grain counting in autoradiography, determination of HLA genotype, electrophoretic gels analysis, automatic screening of cervical smears.
Thus several heterogeneous applications may share the same image processing device, provided there is a separate and devoted work station for each of them. Doty, Charles R. Major resources exist that can be used to develop or upgrade programs in community colleges and technical institutes that educate robotics/automated systems technicians.
The first category of resources is Economic, Social, and Education Issues. The Office of Technology Assessment (OTA) report, ' Automation and the Workplace,' presents analyses of. Doty, Charles R. Major resources exist that can be used to develop or upgrade programs in community colleges and technical institutes that educate robotics/automated systems technicians. The first category of resources is Economic, Social, and Education Issues. The Office of Technology Assessment (OTA) report, ' Automation and the Workplace,' presents analyses of. Andreyewsky, Alexander 1981-01-01 Examines electronic aids to translation both as ways to automate it and as an approach to solve problems resulting from shortage of qualified translators.
Describes the limitations of robotic MT (Machine Translation) systems, viewing MAT (Machine-Aided Translation) as the only practical solution and the best vehicle for further automation. (MES). Wille, T; Thiermann, H; Worek, F 2010-04-01 Developing improved medical countermeasures against chemical warfare agents (nerve agents) is urgently needed but time-consuming and costly.
Here we introduce a robot-assisted liquid handling system with warming, cooling and incubating facilities to screen the detoxifying properties of biological and chemical materials against nerve agents. Two biological tests were established and plasma from various species, DFPase and three cyclodextrins were used as test materials. In test 1, plasma was mixed with sarin or VX and the inhibitory potency of the incubate was determined with human acetylcholinesterase (AChE) at 0, 30 and 60 min. In test 2, test materials and nerve agents were mixed and incubated. Between 0 and 40 min samples were taken and incubated for 3 min with AChE and the residual AChE inhibition was determined to enable the semi-quantitative evaluation of the detoxification kinetics. The automated assays proved to be highly reproducible. It was possible to pre-select detoxifying reagents with test 1 and to determine more detailed detoxifying kinetics with test 2.
In conclusion, the automated assay may be considered as a versatile tool for the high-throughput screening of potential detoxifying materials against different nerve agents. With this two-step assay it is possible to screen effectively for detoxifying materials in a high-throughput system. Copyright (c) 2009 Elsevier Ltd. All rights reserved.
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Berry, William F.; Giarrocco, Vince 1994-01-01 An automated method, based on the Hewlett-Packard ORCA (Optimized Robot for Chemical Analysis) system, for sample preparation and analysis of petroleum samples by simulated distillation (SIMDIS) is described. Results obtained for the robotically prepared samples show excellent agreement with those obtained from the same samples prepared manually. The application, based on ASTM method D 2887, is the foundation for a more fully automated system that can perform a variety of SIMDIS samples and methods. PMID:18924992. Wallerstein, D. V.; Haggenmacher, G. 1976-01-01 An automated strength sizing capability is described.
The technique determines the distribution of material among the elements of a structural model. The sizing is based on either a fully stressed design or a scaled feasible fully stressed design. Results obtained from the application of the strength sizing to the structural sizing of a composite material wing box using material strength allowables are presented. These results demonstrate the rapid convergence of the structural sizes to a usable design.
Triversa Nanomate
D.; Yu, G.; Bibby, M.; Dunn, C.; Li, H.; Wu, Y.; Zheng, X.; Zhang, P. 2016-02-01 We first present a Case Study - the manufacture of 1.4 m prototype mirror-segments for the European Extremely Large Telescope, undertaken by the National Facility for Ultra Precision Surfaces, at the OpTIC facility operated by Glyndwr University.
Scale-up to serial-manufacture demands delivery of a 1.4 m off-axis aspheric hexagonal segment with surface precision.
The coupling of reduced liquid chromatography (LC) flow rates with electrospray ionization (ESI) provides enhanced sensitivity and ionization efficiency, and reduced ion suppression in mass spectrometry analysis. As the LC flow increases, the ionization efficiency drops owing to the larger sized droplets that lowers the number of available charges per analyze molecule. In order to accommodate micro-flow rates (defined as 1-10μL/min), we have developed a silicon-based ESI chip with various I.D. Sizes (4.1, 5.5, 9.5, and 13.5μm).
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